ELISA Sheep Interleukin 2 (IL-2)

Reactivity:Sheep (Ovis aries) UniProt:P19114 Abbreviation:IL2 Alternative Names:IL-2; TCGF; lymphokine; T cell growth factor|aldesleukin|interleukin-2|involved in regµLation of T-cell clonal expansion Application:ELISA Range:1.56-100 ng/mL Sensitivity:0.51 ng/mL Intra-AssayCV:?5.5% Inter-AssayCV:?11.2% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate IL2 in samples. An antibody specific for IL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for IL2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Interleukin 2 (IL-2) is a pleiotropic cytokine produced primarily by mitogen- or antigen-activated T lymphocytes. IL-2 plays a key role in promoting the clonal expansion of antigen-specific T cells. In addition, IL-2 has also been shown to mediate mµLtiple immune responses on a variety of cell types. IL-2 stimµLates the proliferation of thymocytes; stimµLates the proliferation and differentiation of activated B cells; promotes the growth, differentiation and cytocidal activity of monocytes; induces the growth of and cytokine production and cytolytic activity by natural killer cells; enhances the production of lymphocyte-activated killer (LAK) cells; and induces the proliferation and differentiation of oligodendrocytes Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).