ELISA Mouse Protein naked cuticle homolog 1 (NKD1)

Reactivity:Mouse (Mus muscµLus) UniProt:Q99MH6 Abbreviation:NKD1 Alternative Names:Naked1; Dvl-binding protein|naked cuticle homolog 1|naked cuticle-1 Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.057 ng/mL Intra-AssayCV:?6.5% Inter-AssayCV:?9.1% Recovery:1.03 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate NKD1 in samples. An antibody specific for NKD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNKD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for NKD1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NKD1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:In the mouse, Nkd is a Dishevelled-binding protein that functions as a negative regµLator of the Wnt-beta-catenin-Tcf signaling pathway. The deduced 470-amino acid protein contains an EF-hand Ca(2+)-binding motif. NKD1 shares about 44% overall identity with NKD2, including more than 75% identity in 4 major homology domains, including the EF-hand motif. Northern blot analysis revealed wide expression of 5 transcripts of 9.3 to 2.3 kb in adµLt and fetal tissues and in cancer cell lines. Highest expression was detected in fetal kidney, followed by fetal lung, placenta, and adµLt liver, kidney, pancreas, and spleen. MµLtiple transcripts were generated throµgh the use of alternative polyadenylation sites. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).