ELISA Semaphorin-3C (SEMA3C)

Reactivity: (Homo sapiens) UniProt:Q99985 Abbreviation:SEMA3C Alternative Names:SEMAE; SemE; OTTHUMP00000207932|semaphorin 3C|semaphorin E Application:ELISA Range:15.6-1000 pg/mL Sensitivity:6.8 pg/mL Intra-AssayCV:?6.9% Inter-AssayCV:?8.8% Recovery:1.04 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SEMA3C in samples. An antibody specific for SEMA3C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA3C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SEMA3C is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA3C bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:The 616-amino acid semaphorin E contains an N-terminal signal sequence of 15 amino acids that is followed by a semaphorin domain. In vitro translation of the semaphorin E cDNA yielded a 70-kD protein. The authors showed that semaphorin E is secreted by mammalian cells. By Northern blot analysis, semaphorin E was expressed as a 5.2-kb transcript in all tissues tested. CDDP-sensitive cells transfected with semaphorin E cDNA acquired CDDP-resistance. Semaphorin E was overexpressed in CDDP-resistant cell lines and was induced by diverse chemotherapeutic drµgs and by x-ray and UV irradiation. Aberrant semaphorin E protein expression was detected immunohistochemically Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).