ELISA Mouse Creatinine (Cr)

Reactivity:Mouse (Mus muscµLus) UniProt:N/A Abbreviation:Cr Alternative Names:N/A Application:ELISA Range:0.156-10 µg/mL Sensitivity:0.1 µg/mL Intra-AssayCV:?4.6% Inter-AssayCV:?7.8% Recovery:0.9 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate Cr in samples. An antibody specific for Cr has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCr present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for Cr is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Cr bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Creatinine is a breakdown product of creatine phosphate in muscle, and is usually produced at a fairly constant rate by the body. Serum creatinine is an important indicator of renal health because it is an easily measured byproduct of muscle metabolism that is excreted unchanged by the kidneys. Creatinine itself is produced via a biological system involving creatine, phosphocreatine, and adenosine triphosphate. Creatine is syntheQuantityd primarily in the liver from the methylation of glycocyamine (guanidino acetate, syntheQuantityd in the kidney from the amino acids arginine and glycine) by S-adenosyl methionine. It is then transported throµgh blood to the other organs, muscle, and brain, where, throµgh phosphorylation, it becomes the high-energy compound phosphocreatine. During the reaction, creatine and phosphocreatine are catalyzed by creatine kinase, and a spontaneous conversion to creatinine may occur. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).