ELISA Paraneoplastic antigen Ma2 (PNMA2)

Reactivity: (Homo sapiens) UniProt:Q9µL42 Abbreviation:PNMA2 Alternative Names:KIAA0883; MA2; MM2; RGAG2; onconeuronal antigen MA2|paraneoplastic neuronal antigen|retrotransposon gag domain containing 2 Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.058 ng/mL Intra-AssayCV:?6.3% Inter-AssayCV:?9.1% Recovery:1 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PNMA2 in samples. An antibody specific for PNMA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPNMA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PNMA2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PNMA2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:In patients with cancer, symptoms of limbic and brainstem dysfunction may resµLt from a paraneoplastic disorder. Paraneoplastic limbic or brainstem encephalitis occurs more frequently with testicµLar cancer than with most other cancers. By immunohistochemical and immunoblotting techniques, Voltz et al. (1999) detected antibodies in serum and cerebrospinal fluid against a 40-kD neuronal protein in 10 of 13 patients with testicµLar cancer and paraneoplastic limbic or brainstem encephalitis, or both. They designated the protein MA2, and found that it was selectively expressed by normal brain tissue and by the testicµLar tumors of the patients. MA2 shares homology with MA1, a 'brain testis-cancer' gene related to other neoplastic syndromes and tumors. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).