ELISA GTP-binding protein Di-Ras3 (DIRAS3)

Reactivity: (Homo sapiens) UniProt:O95661 Abbreviation:DIRAS3 Alternative Names:ARHI; NOEY2; OTTHUMP00000011138|ras homolog gene family; member I Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.3% Inter-AssayCV:?9.8% Recovery:1.03 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate DIRAS3 in samples. An antibody specific for DIRAS3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDIRAS3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DIRAS3 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DIRAS3 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:ARHI contains a highly conserved GTP-binding domain, a putative effector domain distinct from that of RAS and RAP proteins, and a C-terminal membrane localization motif. Northern blot analysis detected a 1.9-kb ARHI transcript in all normal breast and ovarian epithelial cell cµLtures tested, as well as in normal ovary, heart, liver, pancreas, and brain. Expression was absent in nearly all breast and ovarian cancer cell lines and all primary ovarian cancer cell lines tested. Western blot analysis detected a 26-kD ARHI protein in all normal breast and ovarian cell lines but not in any breast and ovarian cancer cell lines tested. Expression of ARHI in breast and ovarian cancer cell lines but not in lung cancer cell lines led to growth inhibition. StimµLation of normal cell lines with growth factors led to decreased expression of ARHI as well as the cell growth inhibition-associated protein WAF1. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).