ELISA Mouse Dystrophin (DMD)

Reactivity:Mouse (Mus muscµLus) UniProt:P11531 Abbreviation:DMD Alternative Names:GS1-19O24.1; BMD; CMD3B; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.123 ng/mL Intra-AssayCV:?5.3% Inter-AssayCV:?9.2% Recovery:1.01 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate DMD in samples. An antibody specific for DMD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DMD is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMD bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Dystrophin is a rod-shaped cytoplasmic protein, and a vital part of a protein complex that connects the cytoskeleton of a muscle fiber to the surrounding extracellµLar matrix throµgh the cell membrane. This complex is variously known as the costamere or the dystrophin-associated protein complex. Many muscle proteins, such as ?-dystrobrevin, syncoilin, synemin, sarcoglycan, dystroglycan, and sarcospan, colocalize with dystrophin at the costamere. The DMD gene, encoding the dystrophin protein, is one of the longest genes known, covering 2.3 megabases (0.08% of the genome) at locus Xp21. The primary transcript in muscle measures about 2,100 kilobases and takes 16 hours to transcribe; the mature mRNA measures 14.0 kilobases. The 79-exon muscle transcript[5] codes for a protein of 3685 amino acid residues. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).