ELISA Dynamin 2 (DNM2)

Reactivity: (Homo sapiens) UniProt:P50570 Abbreviation:DNM2 Alternative Names:CMTDI1; CMTDIB; DI-CMTB; DYN2; DYNII; dynamin II Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.156 ng/mL Intra-AssayCV:?6.9% Inter-AssayCV:?8.4% Recovery:1.01 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate DNM2 in samples. An antibody specific for DNM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDNM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DNM2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNM2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecµLe, which contains the GTPase domain. Dynamins are associated with microtubµLes. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimµLates GTPase activity. Four alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their fµLl-length nature has not been determined. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).