ELISA Dentin sialophosphoprotein (DSPP)

Reactivity: (Homo sapiens) UniProt:Q9NZW4 Abbreviation:DSPP Alternative Names:DFNA39; DGI1; DMP3; DPP; DSP; DTDP2; dentin phosphophoryn|dentin phosphoprotein|dentin phosphoryn|dentin sialoprotein Application:ELISA Range:1.56-100 ng/mL Sensitivity:0.78 ng/mL Intra-AssayCV:?4.3% Inter-AssayCV:?7.8% Recovery:0.88 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate DSPP in samples. An antibody specific for DSPP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDSPP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DSPP is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DSPP bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Dentin sialophosphoprotein, is the only protein produced uniquely by odontoblasts, the cells that produce tooth dentin. It is a non-collagenous SIBLING protein that is later cleaved into three functional proteins: dentin phosphoprotein (also known as phosphophoryn), taken from the C-terminal end, dentin sialoprotein from the N-terminal end, and dentin glycoprotein from the middle of the molecµLe. Their distribution in the collagen matrix of the forming dentin sµggests these proteins play an important role in the regµLation of mineral deposition. Additional evidence for this correlation is phenotypically manifested in patients with mutant forms of dentin sialophosphoprotein. Such patients suffer dental anomalies including type III dentinogenesis imperfecta. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).