ELISA Beta-klotho (KLB)

Reactivity: (Homo sapiens) UniProt:Q86Z14 Abbreviation:KLB Alternative Names:BKL; MGC142213; klotho beta like Application:ELISA Range:78.1-5000 pg/mL Sensitivity:29 pg/mL Intra-AssayCV:?4.3% Inter-AssayCV:?8.1% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate KLB in samples. An antibody specific for KLB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for KLB is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLB bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:KLb encodes a 1,043-amino acid protein that shares 41.2% identity with mouse Kl. Like Kl, Klb contains an N-terminal signal sequence, followed by 2 tandemLy repeated glycosidase-like domains, a C-terminal transmembrane domain, and a short cytoplasmic tail. Both Kl and Klb lack 2 key glutamates in the predicted active centers of the glycosidase-like domains. Northern blot analysis of adµLt mouse tissues showed that Klb was predominantly expressed in liver and pancreas, with lower levels in skin and weak expression in stomach, skeletal muscle, small intestine, and lung. In mouse embryos, Klb expression became detectable at day 11 and increased in intensity as development progressed. In situ hybridization of mouse embryos revealed strong Klb expression in yolk sac, gut, brown and white adipose tissue, liver, and pancreas. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).