ELISA Keratin, type I cytoskeletal 40 (KRT40)

Reactivity: (Homo sapiens) UniProt:A7YWM2 Abbreviation:KRT40 Alternative Names:FLJ36600; KA36; OTTHUMP00000164963|type I hair keratin KA36 Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.064 ng/mL Intra-AssayCV:?5.2% Inter-AssayCV:?8.9% Recovery:0.89 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate KRT40 in samples. An antibody specific for KRT40 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT40 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for KRT40 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT40 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:KRT40 encodes a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubµLes, compose the cytoskeleton of epithelial cells. The type I keratin genes are clustered in a region of chromosome 17q12-q21. Keratins are intermediate filament-forming proteins that provide mechanical support and fµLfill a variety of additional functions in epithelial cells. In 1982, a nomenclature was devised to name the keratin proteins that were known at that point. The systematic sequencing of the genome in recent years uncovered the existence of several novel keratin genes and their encoded proteins. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).