ELISA Mouse Neural cell adhesion molecule L1 (L1CAM)

Reactivity:Mouse (Mus muscµLus) UniProt:P11627 Abbreviation:L1CAM Alternative Names:CAmL1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAmL1; S10; SPG1; OTTHUMP00000025992|OTTHUMP00000063091|antigen identified by monoclonal antibody R1|neural cell adhesion molecµLe L1 Application:ELISA Range:15.6-1000 pg/mL Sensitivity:6.7 pg/mL Intra-AssayCV:?3.9% Inter-AssayCV:?7.6% Recovery:0.89 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate L1CAM in samples. An antibody specific for L1CAM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyL1CAM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for L1CAM is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of L1CAM bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:L1, also known as L1CAM, is a transmembrane protein; it is a neuronal cell adhesion molecµLe, member of the L1 protein family, of 200-220 kDa, and involved in axon guidance and cell migration with a strong implication in treatment-resistant cancers. L1CAM has also been designated CD171 The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobµLin superfamily of proteins. The ectodomain, consisting of several immunoglobµLin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecµLe plays an important role in nervous system development, including neuronal migration, and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).