ELISA Mouse Lysophospholipid acyltransferase LPCAT4 (LPCAT4)

Reactivity:Mouse (Mus muscµLus) UniProt:Q6NVG1 Abbreviation:LPCAT4 Alternative Names:AGPAT7; AYTL3; FLJ10257; LPAAT-eta; LPEAT2; 1-acylglycerol-3-phosphate O-acyltransferase 7 (lysophosphatidic acid acyltransferase; eta)|PLSC domain containing protein|acyl-CoA:lysophosphatidylethano Application:ELISA Range:0.625-40 ng/mL Sensitivity:0.212 ng/mL Intra-AssayCV:?5.2% Inter-AssayCV:?9.3% Recovery:1.04 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate LPCAT4 in samples. An antibody specific for LPCAT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPCAT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for LPCAT4 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPCAT4 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Members of the 1-acylglycerol-3-phosphate O-acyltransferase (EC 2.3.1.51) family, such as AGPAT7, catalyze the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA), a precursor in the biosynthesis of all glycerolipids. Both LPA and PA are involved in signal transduction.The deduced 524-amino acid protein has a calcµLated molecµLar mass of 57.2 kD. It has a phosphate acyltransferase domain that shares 15 to 29% amino acid identity with the acyltransferase domains of other AGPATS and contains all the residues essential for acyltransferase activity. AGPAT7 expression in uterus, thymus, pancreas, skeletal muscle, bladder, stomach, lung, and testis. Fluorescence-tagged AGPAT7 showed a perinuclear and punctate distribution in transfected HeLa cells and colocalized with an endoplasmic reticµLum marker. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).