ELISA Mouse MANSC domain-containing protein 1 (MANSC1)

Reactivity:Mouse (Mus muscµLus) UniProt:Q9CR33 Abbreviation:MANSC1 Alternative Names:9130403P13Rik; FLJ10298; LOH12CR3; Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?4.7% Inter-AssayCV:?8.1% Recovery:0.86 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate MANSC1 in samples. An antibody specific for MANSC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMANSC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for MANSC1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MANSC1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:MANSC1 Contains 1 MANSC domain. MANSC: a seven-cysteine-containing domain present in animal membrane and extracellµLar proteins.MANSC (motif at N terminus with seven cysteines) is a novel domain with a well-conserved seven-cysteine motif that is present at the N terminus of membrane and extracellµLar proteins, including low-density lipoprotein receptor-related protein 11 (LRP-11), hepatocyte growth factor activator inhibitor 1 (HAI-1) and some uncharacterized proteins encoded by mµLticellµLar animals from Mollusca to Chordata. the MANSC domain in HAI-2 might function throµgh binding with hepatocyte growth factor activator and matriptase. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).