ELISA Small Tailed Han Sheep Proopiomelanocortin (POMC)

Reactivity:Small Tailed Han Sheep (Ovis aries) UniProt:N/A Abbreviation:POMC Alternative Names:ACTH; CLIP; LPH; MSH; NPP; POC; OTTHUMP00000119991|OTTHUMP00000200964|adrenocorticotropic hormone|adrenocorticotropin|alpha-MSH|alpha-melanocyte-stimµLating hormone|beta-LPH|beta-MSH|beta-endorphin| Application:ELISA Range:7.8-500 pg/mL Sensitivity:1.95 pg/mL Intra-AssayCV:?4.7% Inter-AssayCV:?7.5% Recovery:1.02 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate POMC in samples. An antibody specific for POMC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOMC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for POMC is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POMC bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Pro-opiomelanocortin (POMC) is a precursor polypeptide with 241 amino acid residues. This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellµLar functions. The encoded protein is syntheQuantityd mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).