ELISA Serine|threonine-protein phosphatase 2B catalytic subunit beta isoform (PPP3CB)

Reactivity: (Homo sapiens) UniProt:P16298 Abbreviation:PPP3CB Alternative Names:RP11-345K20.1; CALNA2; CALNB; CNA2; CAM-PRP catalytic subunit|calcineurin A beta|calcineurin A2|calmodµLin-dependent calcineurin A subunit beta|protein phosphatase 3; catalytic subunit; beta|protein Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.064 ng/mL Intra-AssayCV:?5.8% Inter-AssayCV:?10.8% Recovery:0.89 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PPP3CB in samples. An antibody specific for PPP3CB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPP3CB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PPP3CB is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPP3CB bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Calcineurin, a calmodµLin-regµLated protein phosphatase, is found in the cells of all eukaryotes ranging from yeast to mammals. Wang et al. (1996) described this heterodimeric protein as having a 19-kD Ca(2+)-binding regµLatory subunit, calcineurin B, and a 58- to 59-kD catalytic subunit, calcineurin A. One gene encodes calcineurin B (PPP3R1) in all tissues except testis, and it is highly conserved at the level of both protein and DNA sequences in eukaryotes. In contrast, there are 2 major isoforms, alpha (PPP3CA) and beta (PPP3CB), of calcineurin A encoded by separate genes located on different chromosomes. A third isoform, A-gamma (PPP3CC), is unique to testis. Additional diversity of calcineurin A is created by alternative splicing of mRNAs. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).