ELISA Pre-mRNA-processing factor 6 (PRPF6)

Reactivity: (Homo sapiens) UniProt:O94906 Abbreviation:PRPF6 Alternative Names:ANT-1; C20orf14; TOM; U5-102K; hPrp6; PRP6 pre-mRNA processing factor 6 homolog|U5 snRNP-associated 102 kDa protein|androgen receptor N-terminal domain transactivating protein-1|p102 U5 small nuclea Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.078 ng/mL Intra-AssayCV:?4.6% Inter-AssayCV:?8.3% Recovery:0.88 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PRPF6 in samples. An antibody specific for PRPF6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRPF6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PRPF6 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRPF6 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:PRP6 appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing. Predicted amino acid sequence of the protein is similar to those of yeast splicing factors, Prp1p/Zer1p of Schizosaccharomyces pombe and Prp6p of Saccharomyces cerevisiae. Among these proteins, tetratrico peptide repeat (TPR) motif, which mediates protein-protein interactions, is conserved, whereas leucine zipper motif is found only in the 100-kDa protein. Indirect immunofluorescent staining showed that the 100-kDa protein localized in the nucleus in HeLa cells. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).