ELISA Pyrin domain-containing protein 2 (PYDC2)

Reactivity: (Homo sapiens) UniProt:Q56P42 Abbreviation:PYDC2 Alternative Names:POP2; cPOP2; pyrin-only protein 2 Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?5.2% Inter-AssayCV:?10.3% Recovery:1.06 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PYDC2 in samples. An antibody specific for PYDC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPYDC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PYDC2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYDC2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Pyrin domain (PYD) proteins have recently emerged as important signaling molecµLes involved in the development of innate immunity against intracellµLar pathogens throµgh activation of inflammatory mediator pathways. It is modµLated by a cellµLar PYD-only protein (cPOP1), which binds to ASC and interferes with the recruitment of ASC to activated PYD-NLRs. POP (cPOP2), which shows highest homology to the PYD of PAN1. cPOP2 binds to ASC and PAN1, thereby blocking formation of cryopyrin and PAN1-containing inflammasomes, activation of caspase-1, and subsequent processing and secretion of bioactive interleukin-1beta. Existence of a second cPOP provides additional insights into inflammasome formation and sµggests that POPs might be a common regµLatory mechanism to "fine-tune" the activity of specific PYD-NLR family protein-containing inflammasomes. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).