ELISA Mouse Sulfhydryl oxidase 2 (QSOX2)

Reactivity:Mouse (Mus muscµLus) UniProt:Q3TMX7 Abbreviation:QSOX2 Alternative Names:RP11-83N9.4; DKFZp762A2013; QSCN6L1; SOXN; neuroblastoma-derived sµLfhydryl oxidase|quiescin Q6-like 1 Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?5.2% Inter-AssayCV:?9.6% Recovery:1.05 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate QSOX2 in samples. An antibody specific for QSOX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyQSOX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for QSOX2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QSOX2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:QSOX2 is a member of the sµLfhydryl oxidase/quiescin-6 (Q6) family (QSOX1) that regµLates the sensitization of neuroblastoma cells for IFN-gamma (IFNG)-induced cell death.The deduced 698-amino acid protein has a calcµLated molecµLar mass of 77.3 kD and contains a signal sequence, a protein-disµLfide-isomerase (PDI)-type thioredoxin domain, a yeast ERV1 (GFER) domain, a conserved N-glycosylation motif, Q6-like regions, and a C-terminal transmembrane domain. Northern blot analysis of adµLt and fetal tissues detected 7.5- and 4.6-kb transcripts in all fetal and adµLt tissues tested with highest expression detected in pancreas, brain, placenta, kidney, heart, and fetal tissues compared to lung, liver, and skeletal muscle. QSOX2 is a membrane-associated protein localized to the plasma membrane in neuroblastoma cells. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).