ELISA Mouse Adenylate cyclase type 10 (ADCY10)

Reactivity:Mouse (Mus muscµLus) UniProt:Q8C0T9 Abbreviation:ADCY10 Alternative Names:HCA2; RP1-313L4.2; SAC; SACI; Sacy; 3 ;5 -cyclic AMP synthetase|AH-related protein|ATP pyrophosphate-lyase|adenylate cyclase 10|testicµLar soluble adenylyl cyclase (SAC) Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.132 ng/mL Intra-AssayCV:?4.8% Inter-AssayCV:?7.9% Recovery:0.89 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate ADCY10 in samples. An antibody specific for ADCY10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADCY10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for ADCY10 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADCY10 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Adenylate cyclase type 10 is a protein encoded by this gene belongs to a distinct class of mammalian adenylyl cyclase that is soluble in testis but particµLate in sperm and other tissues. It is unlike other mammalian adenylyl cyclases because it is insensitive to G protein or forskolin regµLation and is directly regµLated by bicarbonate. It is localized in the cytoplasm and to organelles and is thoµght to function as a general bicarbonate sensor throµghout the body. It plays an important role in the generation of cAMP in spermatozoa, playing possible roles in sperm capacitation, hypermotility, and/or the acrosome reaction.Genetic variations in this gene are associated with absorptive hypercalciuria type 2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).