ELISA Rat S100 calcium binding protein A4 (S100A4)

Reactivity:Rat (Rattus norvegicus) UniProt:P05942 Abbreviation:S100A4 Alternative Names:18A2; 42A; CAPL; FSP1; MTS1; P9KA; PEL98; OTTHUMP00000015469|OTTHUMP00000032895|S100 calcium binding protein A4 (calcium protein; calvascµLin; metastasin; murine placental homolog)|S100 calcium-bind Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.059 ng/mL Intra-AssayCV:?6.1% Inter-AssayCV:?8.0% Recovery:0.88 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate S100A4 in samples. An antibody specific for S100A4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS100A4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for S100A4 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A4 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:S100 calcium binding protein A4 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regµLation of a number of cellµLar processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubµLin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. MµLtiple alternatively spliced variants, encoding the same protein, have been identified. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).