ELISA Sheep Serum Amyloid A (SAA)

Reactivity:Sheep (Ovis aries) UniProt:P42819 Abbreviation:SAA1 Alternative Names:MGC111216; PIG4; SAA; TP53I4; tumor protein p53 inducible protein 4 Application:ELISA Range:78.1-5000 ng/mL Sensitivity:40 ng/mL Intra-AssayCV:?6.3% Inter-AssayCV:?9.3% Recovery:0.92 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SAA1 in samples. An antibody specific for SAA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySAA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SAA1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SAA1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Serum amyloid A protein is a protein encoded by the SAA1 gene.The serum amyloid A (SAA) protein is an acute phase apolipoprotein reactant produced mainly by hepatocytes and under regµLation of inflammatory cytokines (summarized by Lundmark et al., 2002). The SAA cleavage product, designated amyloid protein A (AA), is deposited systemically as amyloid in vital organs including the liver, spleen, and kidneys in patients with chronic inflammatory diseases. There is very limited structural information about SAA because of its inherent poor solubility in the apolipoprotein form. It is intriguing to understand how such a small protein is able to mediate or directly carry out such a wide range of functions related to inflammatory reaction and other hostdefense mechanisms. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).