ELISA Serum deprivation response (SDPR)

Quantity:96T. Other Quantitys are also available. Please Inquire. Reactivity: (Homo sapiens) UniProt:O95810 Abbreviation:SDPR Alternative Names:PS-p68; SDR; cavin-2; phosphatidylserine binding protein|serum deprivation response (phosphatidylserine-binding protein)|serum deprivation response protein Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.054 ng/mL Intra-AssayCV:≤6.1% Inter-AssayCV:≤10.2% Recovery:0,97 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SDPR in samples. An antibody specific for SDPR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySDPR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SDPR is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDPR bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:SDPR encodes a calcium-independent phospholipid-binding protein whose expression increases in serum-starved cells. This protein has also been shown to be a substrate for protein kinase C (PKC) phosphorylation. The SDPR cDNA encodes a deduced 425-amino acid peptide with a calcµLated molecµLar mass of 47.2 kD. The and mouse proteins share 84% sequence identity; both contain a leucine zipper-like domain with 7 repeats and 2 putative protein kinase C phosphorylation sites. Northern blot analysis of various tissues showed nearly ubiquitous expression of a 3.1-kb transcript, which was always coexpressed with a shorter transcript. Highest expression was detected in heart and lung.SDPR is able to bind PS liposomes in a calcium-independent manner. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).