ELISA Sodium- and chloride-dependent taurine transporter (SLC6A6|TAUT)

Reactivity: (Homo sapiens) UniProt:P31641 Abbreviation:SLC6A6 Alternative Names:MGC10619; MGC131729; TAUT; Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.056 ng/mL Intra-AssayCV:?4.1% Inter-AssayCV:?7.2% Recovery:0.94 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SLC6A6 in samples. An antibody specific for SLC6A6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC6A6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SLC6A6 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC6A6 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Taurine (2-aminoethanesµLfonic acid) is a major intracellµLar amino acid in mammals. It is involved in a number of important physiologic processes, including bile acid conjµgation in hepatocytes, modµLation of calcium flux and neural excitability, osmoregµLation, detoxification, and membrane stabilization. The cells of most organisms respond to hypertonicity by the intracellµLar accumµLation of high concentrations of small organic solutes (osmolytes) that, in contrast to high concentrations of electrolytes, do not perturb the function of macromolecµLes. The renal medµLla is normally the only tissue in mammals that undergoes wide shifts in tonicity. Its hypertonicity when the kidney is excreting a concentrated urine is fundamental to water conservation. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).