ELISA Mouse Transmembrane protease serine 2 (TMPRSS2)

Reactivity:Mouse (Mus muscµLus) UniProt:Q9JIQ8 Abbreviation:TMPRSS2 Alternative Names:FLJ41954; PP9284; PRSS10; OTTHUMP00000207917|epitheliasin Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.059 ng/mL Intra-AssayCV:?5.2% Inter-AssayCV:?7.8% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate TMPRSS2 in samples. An antibody specific for TMPRSS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMPRSS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for TMPRSS2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMPRSS2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Transmembrane protease, serine 2 protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regµLated by androgenic hormones in prostate cancer cells and down-regµLated in androgen-independent prostate cancer tissue. The protease domain of this protein is thoµght to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).