ELISA Vascular cell adhesion molecule 1 (VCAM-1)

Reactivity:Pig (Sus scrofa; Porcine) UniProt:N/A Abbreviation:VCAM1/CD106 Alternative Names:CD106; INCAM-100 Application:ELISA Range:78.1-5000 pg/mL Sensitivity:30 pg/mL Intra-AssayCV:?4.6% Inter-AssayCV:?10.6% Recovery:1.09 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate VCAM1/CD106 in samples. An antibody specific for VCAM1/CD106 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVCAM1/CD106 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for VCAM1/CD106 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VCAM1/CD106 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:VCAM-1 (CD106), a member of the immunoglobµLin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1?, IL-4, TNF-? and IFN-?. VCAM-1 binds to leukocyte integrins VLA-4 and ?4?7. The rat and mouse SVCAM-1 proteins share approximately 76% amino acid similarity. VCAM-1 is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).