Saccharomyces cerevisiae Surface Display Vector Kit -1 Kit
The Saccharomyces cerevisiae Surface Display Vector Kit is a complete molecular toolkit designed for the efficient display of heterologous proteins or peptides on the surface of yeast cells. Utilizing the robust expression system of S. cerevisiae, this kit allows researchers to fuse target genes to cell wall-anchoring motifs, enabling high-throughput screening, affinity selection, and protein engineering directly on the yeast surface.
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What is Saccharomyces cerevisiae ?
Saccharomyces cerevisiae is well suited to cell surface display as a common cell factory for the production of various fuels and chemicals, with the advantages of large cell size, being a Generally Regarded As Safe (GRAS) organism, and post-translational processing of secreted proteins.
Applications:
- Protein-protein and protein-ligand interaction studies
- Antibody library screening and affinity maturation
- Epitope mapping and vaccine target validation
- Enzyme engineering and directed evolution
- Biosensor development and surface-binding assays
Kit Contents:
- Yeast surface display expression vector(s)
- Control insert and yeast transformation protocol
- Selective yeast growth media components
- Detailed user manual with cloning and expression guidance
Key Features:
- High-efficiency yeast surface display using Aga1p/Aga2p or similar anchor systems.
- Customizable vector design with multiple cloning sites for gene-of-interest insertion.
- Strong promoters for high-level expression in S. cerevisiae.
- Selectable markers and yeast-compatible origins for stable maintenance and expression.
- Optimized for library construction, epitope mapping, or antibody-antigen interaction studies.
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