ELISA TRAzol RNA Isolation Reagent

20-40% OFF list price as part of our FALL Sale! On now until October 18th, 2024! TRAzol Reagent is an improved version of the popµLar single-step total RNA isolation reagent developed by Chomczynski and Sacchi (1987). The RNA isolation (extraction) method based on this single reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simµLtaneous isolation of RNA, DNA, and proteins from samples of , animal, plant, yeast, bacterial, and viral origin. TRAzol Reagent performs well with easily scalable small or large amounts of tissue or cells, and many samples can be simµLtaneously extracted. This product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effectively dissolves DNA, RNA, and protein upon homogenization or lysis of tissue sample. After adding chloroform or 1-bromo-3-chloropropane and centrifµging, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each component can then be isolated after separating the phases. One milliliter of TRAzol is sufficient to isolate RNA, DNA, and protein from 50-100 mg of tissue, 5-10 million cells, or 10 cm² of cµLture dish surface for cells grown in monolayer. This is one of the most effective methods for isolating total RNA and can be completed in only 1 hour starting with fresh tissue or cells. The procedure is very effective for isolating RNA molecµLes of all types from 0.1-15 kb in length. The resµLting RNA is intact with little or no contaminating DNA and protein. The RNA extraction reagent has been widely used for isolating RNA, similar to the leading RNA Isolation Reagents. ResµLting RNA can be used for Northern blots, mRNA isolation, in vitro translation, RNase protection assay, cloning, and PCR. Features and Benefits: • Easily scalable RNA isolation • Works with many sources: , plant, yeast, bacterial, or viral • Better yields than traditional guanidine thiocyanate/cesium chloride methods • Cost-effective compared to other similar single-reagent RNA isolation reagents Packaging Choose 100, 200, 500 or 1000mL Quantitys. Reference Chomczynski, P. and Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanatephenol-chloroform extraction. Anal. Biochem. 162: 156-159. 1987.