ELISA FlyCut® NheI

Product Details FlyCut® NheI is expressed and purified from E.coli that carries the recombinant NheI gene. The molecµLar weight is 39.1 kDa, with the recognition site at G^CTAGC. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation. Recognition Site 5’...G↓CTAGC...3’ 3’...CGATC↑G...5’ Applications Genomic DNA, plasmid DNA, PCR product Storage at -20°C for two years Shipping Dry ice (-70 ℃) Product Contents Component JN301-01 JN301-02 FlyCut® NheI 500 units 2×500 units 10×FlyCut® Buffer 250 μl 250 μl 10×DNA Loading Buffer 1 mL 1 mL