SynaptoGreen C4 (also known as FM1-43, a trademark of Molecular Probes, Inc.)

A widely used green fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the red fluorescent dye SynaptoRed (70021), synapses or neuromuscular junctions can be imaged independently in two colours. Research results from the Tsien and Kay labs showed that brain slices can be stained with SynaptoGreen when SR101 (Sulforhodamine 101, 80101) or ADVASEP-7 (70029) is used to reduce the background staining. Biotium offers several Nerve Terminal Staining Kits for staining brain slices. • Ex/ Em: 510/625 nm (in MeoH); 480/598 nm (in membranes) • Red solid soluble in water • C30H49Br2N3 • MW: 612