ELISA TransLv™ Lentivirus qPCR Titration

TransLvTM Lentivirus qPCR Titration Kit provides a simple, rapid, sensitive and efficient method to determine the copy number of integrated proviral sequences in lentivirus transduced cells using qPCR method. Lentivirus titer can be calcµLated from integrated proviral copy number in the genome of the lentivirus transduction susceptible cells (such as HT1080, HEK-293T, etc.) by using the standard curve generated from qPCR. • Simple operation and high sensitivity. • Higher accuracy enabled by internal reference gene. • Compatible with the second and third generation HIV-1 lentiviral packaging vectors. • Good linear relationship in the range of 103-108 copies/μl. Storage at -20°C in the dark for one year. Shipping Dry ice (-70℃) Product Contents Component FV201-01 (100 rxns) 2×TransLvTM Lentivirus qPCR Titration SuperMix 1 mL 10×GC enhancer 200 μl Provirus Gene Standards (P1-P6) (108-103 copies/μl) 30 μl each Reference Gene Standards (R1-R6) (108-103 copies/μl) 30 μl each Provirus Gene Primer Mix (5 μM) 100 μl Reference Gene Primer Mix (5 μM) 100 μl Passive Reference Dye（50×） 40 μl Nuclease-free Water 1 mL