ELISA TransDetect® Double-Luciferase Reporter Assay

Product Details Firefly luciferase and Renilla luciferase can catalyze the oxidation of luciferin or coelenterazine to form oxyluciferin or coelenteramide, respectively, and produce bioluminescence in the process. TransDetect® Double-Luciferase Reporter Assay Kit first uses luciferin as a substrate to detect the activity of the firefly luciferase reporter gene. Then, while quenching the fluorescence reaction, the activity of the Renilla luciferase reporter gene was detected by using coelenterazine as a substrate. It has the characteristics of rapid detection, high sensitivity, wide detection range, and no interference with the endogenous activity of cells. Storage The kit stored at -20°C for one year. Cell Lysis Buffer can be stored at -20°C for one year. Prepared Luciferase Reaction Reagent shoµLd be stored in aliquots in dark at -20°C for one month or at -70°C for one year. Shipping Dry ice (-70℃) Product Contents Component FR201-01-V2 (50 rxns) FR201-02 -V2(200 rxns) Luciferase Reaction Buffer 5 mL 20 mL Luciferase Reaction Substrate (Lyophilized) 1 vial 4 vials Luciferase Reaction Buffer II 5 mL 20 mL Luciferase Reaction Substrate II (50×) 100 μl 400 μl Cell Lysis Buffer (5×) 5 mL 20 mL Citations Zhou W , Yao J , Wang G , et al. PKCζ phosphorylates TRAF2 to protect against intestinal ischemia–reperfusion–induced injury[J]. Cell Death & Disease, 2017, 8(7):e2935. Shan W , Gao L , Zeng W , et al. Activation of the SIRT1/p66shc antiapoptosis pathway via carnosic acid-induced inhibition of miR-34a protects rats against nonalcoholic fatty liver disease[J]. Cell Death and Disease, 2015, 6(7):e1833. Xiong Y , Shi L , Wang L , et al. Activation of sirtuin 1 by catalpol-induced down-regµLation of microRNA-132 attenuates endoplasmic reticµLum stress in colitis[J]. Pharmacological Research, 2017:S1043661817303237. Wang M , Gao Y , Qu P , et al. Sperm-borne miR-449b influences cleavage, epigenetic reprogramming and apoptosis of SCNT embryos in bovine[J]. Scientific Reports, 2017, 7(1):13403. Yang Z , Wang X , Yang J , et al. Aberrant CpG Methylation Mediates Abnormal Transcription ofMAO-AInduced by Acute and Chronicl-3,4-Dihydroxyphenylalanine Administration in SH-SY5Y Neuronal Cells[J]. Neurotoxicity Research, 2017, 31(3):334-347.