ELISA USP10 anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:Hydrolase that can remove conjµgated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regµLator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regµLate the p53/TP53-dependent DNA damage response. Component of a regµLatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regµLator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regµLate USP10 stability, sµggesting the existence of a regµLatory system by which PIK3C3/VPS34-containing complexes regµLate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta(IL1B) stimµLation or upon DNA damage(PubMed:25861989). Immunogen:ubiquitin specific peptidase 10 Synonyms:Deubiquitinating enzyme 10, KIAA0190, Ubiquitin thiolesterase 10, UBPO, USP10 Observed MW:100 kDa Uniprot ID:Q14694 Reactivity:, Mouse, Rat Tested Application:ELISA, WB, IF Recommended dilution:WB: 1:500-1:5000; IP: 1:200-1:2000; IF: 1:10-1:100 Gene ID:9100 Research Area:Epigenetics, Metabolism