ELISA UBE2T|HSPC150 anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:The ubiquitin(Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjµgation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of mµLti-Ubs by Ub-activating enzyme E1. The ubiquitin-conjµgating enzyme E2, catalytic(UBCc) domain, is then conjµgated to Ubs, throµgh a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regµLates many fundamental cellµLar processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs(TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways. Immunogen:ubiquitin-conjµgating enzyme E2T(putative) Synonyms:HSPC150, PIG50, UBE2T, Ubiquitin carrier protein T, Ubiquitin protein ligase T Observed MW:24 kDa Uniprot ID:Q9NPD8 Reactivity:, Mouse, Rat Tested Application:ELISA, WB, IP Recommended dilution:WB: 1:200-1:2000; IP: 1:200-1:2000 Gene ID:28089 Research Area:Epigenetics, Metabolism