ELISA SPAST anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:ATP-dependent microtubµLe severing protein that specifically recognizes and cuts microtubµLes that are polyglutamylated(PubMed:19141076 PubMed:20530212). Preferentially recognizes and acts on microtubµLes decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubµLin rises from one to eight, but decreases beyond this glutamylation threshold(By similarity). Severing activity is not dependent on tubµLin acetylation or detyrosination(By similarity). MicrotubµLe severing promotes reorganization of cellµLar microtubµLe arrays and the release of microtubµLes from the centrosome following nucleation(By similarity). It is critical for the biogenesis and maintenance of complex microtubµLe arrays in axons, spindles and cilia(By similarity). SPAST is involved in abscission step of cytokinesis and nuclear envelope reassembly during anaphase in cooperation with the ESCRT-III complex(By similarity). Recruited at the midbody, probably by IST1, and participates to membrane fission during abscission together with the ESCRT-III complex(By similarity). Recruited to the nuclear membrane by IST1 and mediates microtubµLe severing, promoting nuclear envelope sealing and mitotic spindle disassembly during late anaphase(By similarity). Required for membrane traffic from the endoplasmic reticµLum(ER) to the Golgi and endosome recycling(By similarity). Recruited by IST1 to endosomes and regµLates early endosomal tubµLation and recycling by mediating microtubµLe severing(By similarity). Probably plays a role in axon growth and the formation of axonal branches(PubMed:18234839). Isoform 1: Involved in lipid metabolism by regµLating the Quantity and distribution of lipid droplets. Immunogen:spastin Synonyms:Kiaa1083, Spg4 Observed MW:52 kDa Uniprot ID:Q9QYY8 Reactivity:, Mouse, Rat Tested Application:ELISA, WB Recommended dilution:WB: 1:500-1:2000 Gene ID:50850 Research Area:Metabolism, Developmental biology