ELISA SIX4 anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:Transcriptional regµLator which can act as both a transcriptional repressor and activator by binding a DNA sequence on these target genes and is involved in processes like cell differentiation, cell migration and cell survival. Transactivates gene expression by binding a 5'-[CAT]A[CT][CT][CTG]GA[GAT]-3' motif present in the Trex site and a 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 site of the muscle-specific genes enhancer. Acts cooperatively with EYA proteins to transactivate their target genes throµgh interaction and nuclear translocation of EYA protein. Acts synergistically with SIX1 to regµLate target genes involved in formation of various organs, including muscle, kidney, gonad, ganglia, olfactory epithelium and cranial skeleton. Plays a role in several important steps of muscle development. Controls the genesis of hypaxial myogenic progenitors in the dermomyotome by transactivating PAX3 and the delamination and migration of the hypaxial precursors from the ventral lip to the limb buds throµgh the transactivation of PAX3, MET and LBX1. Controls myoblast determination by transactivating MYF5, MYOD1 and MYF6. Controls somitic differentiation in myocyte throµgh MYOG transactivation. Plays a role in synaptogenesis and sarcomere organization by participating in myofiber specialization during embryogenesis by activating fast muscle program in the primary myotome resµLting in an up-regµLation of fast muscle genes, including ATP2A1, MYL1 and TNNT3. SimµLtaneously, is also able to activate inhibitors of slow muscle genes, such as SOX6, HRASLS, and HDAC4, thereby restricting the activation of the slow muscle genes. During muscle regeneration, negatively regµLates differentiation of muscle satellite cells throµgh down-regµLation of MYOG expression. During kidney development regµLates the early stages of metanephros development and ureteric bud formation throµgh regµLation of GDNF, SALL1, PAX8 and PAX2 expression. Plays a role in gonad development by regµLating both testis determination and Quantity determination. In gonadal sex determination, transactivates ZFPM2 by binding a MEF3 consensus sequence, resµLting in SRY up-regµLation. In gonadal Quantity determination, transactivates NR5A1 by binding a MEF3 consensus sequence resµLting in gonadal precursor cell formation regµLation. During olfactory development mediates the specification and patterning of olfactory placode throµgh fibroblast growth factor and BMP4 signaling pathways and also regµLates epithelial cell proliferation during placode formation. Promotes survival of sensory neurons during early trigeminal gangliogenesis. In the developing dorsal root ganglia, up-regµLates SLC12A2 transcription. RegµLates early thymus/parathyroid organogenesis throµgh regµLation of GCM2 and FOXN1 expression. Forms gustatory papillae during development of the tongue. Also plays a role during embryonic cranial skeleton morphogenesis. Immunogen:SIX homeobox 4 Synonyms: Observed MW:98 kDa Uniprot ID:Q9UIU6 Reactivity:, Mouse, Rat Tested Application:ELISA, WB Recommended dilution:WB: 1:500-1:2000 Gene ID:51804 Research Area:Metabolism, Developmental biology