ELISA SIRT4 anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase(PubMed:19220062). Catalyzes more efficiently removal of lipoyl-and biotinyl-than acetyl-lysine modifications. Inhibits the pyruvate dehydrogenase complex(PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner(PubMed:25525879). Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regµLator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regµLates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest(PubMed:16959573). In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation(PubMed:23663782). Acts as a tumor suppressor(PubMed:23562301, PubMed:23663782). Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of mLYCD, inhibiting its activity, thereby acting as a regµLator of lipid homeostasis(PubMed:23746352). Controls fatty acid oxidation by inhibiting PPARA transcriptional activation. Impairs SIRT1:PPARA interaction probably throµgh the regµLation of NAD(+) levels(PubMed:24043310, PubMed:20685656). Down-regµLates insµLin secretion(By similarity). Immunogen:sirtuin(silent mating type information regµLation 2 homolog) 4(S. cerevisiae) Synonyms:Sir2l4 Observed MW:35 kDa Uniprot ID:Q8R216 Reactivity:, Mouse Tested Application:ELISA, WB, IHC Recommended dilution:WB: 1:500-1:2000; IHC: 1:20-1:200 Gene ID:75387 Research Area:Epigenetics, Metabolism