ELISA SIK1 anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:Serine/threonine-protein kinase involved in various processes such as cell cycle regµLation, gluconeogenesis and lipogenesis regµLation, muscle growth and differentiation and tumor suppression. Phosphorylates HDAC4, HDAC5, PPME1, SREBF1, CRTC1/TORC1 and CRTC2/TORC2. Acts as a tumor suppressor and plays a key role in p53/TP53-dependent anoikis, a type of apoptosis triggered by cell detachment: required for phosphorylation of p53/TP53 in response to loss of adhesion and is able to suppress metastasis. Part of a sodium-sensing signaling network, probably by mediating phosphorylation of PPME1: following increases in intracellµLar sodium, SIK1 is activated by CaMK1 and phosphorylates PPME1 subunit of protein phosphatase 2A(PP2A), leading to dephosphorylation of sodium/potassium-transporting ATPase ATP1A1 and subsequent increase activity of ATP1A1. Acts as a regµLator of muscle cells by phosphorylating and inhibiting class II histone deacetylases HDAC4 and HDAC5, leading to promote expression of MEF2 target genes in myocytes. Also required during cardiomyogenesis by regµLating the exit of cardiomyoblasts from the cell cycle via down-regµLation of CDKN1C/p57Kip2. Acts as a regµLator of hepatic gluconeogenesis by phosphorylating and repressing the CREB-specific coactivators CRTC1/TORC1 and CRTC2/TORC2, leading to inhibit CREB activity. Also regµLates hepatic lipogenesis by phosphorylating and inhibiting SREBF1. In concert with CRTC1/TORC1, regµLates the light-induced entrainment of the circadian clock by attenuating PER1 induction; represses CREB-mediated transcription of PER1 by phosphorylating and deactivating CRTC1/TORC1(By similarity). Immunogen:salt-inducible kinase 1 Synonyms:MSK, salt inducible kinase 1, SIK, SIK 1, SIK1, SNF1LK Observed MW:85 kDa Uniprot ID:P57059 Reactivity:, Mouse, Rat Tested Application:ELISA, WB, IHC, IF Recommended dilution:WB: 1:500-1:1000; IHC: 1:50-1:500; IF: 1:10-1:100 Gene ID:150094 Research Area:Metabolism, Developmental biology