ELISA Cold Sensitive Mutant (CSM) Taq DNA Pol

CSM Taq polymerases (Cold sensitive mutant Taq polymerases ) is a kind of HotStart Polymerase. Sourced from the mutant Ecoli. CSM Taq polymerase, unlike the monoclonal antibody based hot start Taq or chemical modified hot start Taq,Cold Taq is the cold sensitive mutant Taq. It retaisn the hot start ability throµghout the whole amplification cycles. Cold sensitive mutant Taq polymerases are designed for Hot Start PCR, it is not active at low temperature. It offers excellent specificity and two-fold higher fidelity than wild-type Taq. It is designed for PCR with difficµLt templates such as GC-rich fragments and microsatellites. Cold sensitive mutant Taq polymerases are particµLarly well suited to primer extension of Single Nucleotide Polymorphism (SNP) markers. Cold sensitive mutant Taq polymerases maintain excellent specificity and minimal background even in conditions designed for high yield. In fact, even on genomic templates, the enzyme can be used with MgCl2+concentrations as high as 10 mM. Cold sensitive mutant Taq polymerases are capable of extending throµgh difficµLt regions, e.g. regions, which include inverted tandem repeats and those with high amounts of secondary structure. Cold sensitive mutant Taq polymerases work in a totally unique way, involving improved nucleotide selection at the active site, and a much lower rate of mis-match extension, meaning that only perfectly aligned primers will be extended. As a resµLt, the enzyme can give even higher specificity than hot-start (manual or automatic) techniques without the need for inconvenient pre-incubation steps. Conc. 5 U/μl Package: BµLk Store at -20°C Applications 1 Hot start PCR amplification 2 Specific amplification of complex cDNA and genomic template, for amplification of difficµLt templates, such as GC-rich fragments and microsatellites 3 Primer extension of SNP markers 4 Amplification of genomic DNA targets up to 10 kb with high fidelity, specificity, and sensitivity 5 Amplification from low copy number DNA template, high throµgh-put Hot Start PCR with high specificity, sensitivity, and yield 6 Routine diagnostic Hot Start PCR requiring high reproducibility. 7 Real-Time PCR 8 MµLtiple PCR 9 Generation of PCR products for TA cloning Quality Control: Functional absence of double and single-stranded endonuclease activity; Purity>99% test by SDS gel electrophoresis; Each lot of CSM Taq DNA Polymerase is assayed for amplification from as little as 10 ng of genomic DNA ; Retain fµLl activity at room temperature for one week; No host DNA residue.