ELISA Tri-Methyl-Histone H3 (Lys4)|H3K4me3 anti-

Quantity:100µL Host:Rabbit Clonality:Polyclonal Isotype:IgG GeneName:HIST3H3 GeneID:8290 Uniprot:Q16695 Specificity:Tri-Methyl-Histone H3 (Lys4)/H3K4me3 Antibody detects endogenous levels of Tri-Methyl-Histone H3 only when methylated at Lys4. Immunogen:A synthetic methylated peptide derived from Tri-Methyl-Histone H3 around the methylation site of Lys4. Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. Reactivity:,Mouse,Rat Prediction:Horse,Sheep,Rabbit Application:WB,IHC,IF/ICC,IP,ChIP,ELISA(peptide) Dilution:WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:50-1:200, IP 1:50-1:200, CHIP 1:50-1:200, ELISA(peptide) 1:20000-1:40000 Conjµgate:Unconjµgated RRID:AB_2838894 An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site. Cat# Blocking Peptide:DF6935-BP Des# Blocking Peptide:Tri-Methyl-Histone H3 (Lys4)/H3K4me3 Blocking peptide Blocking Peptide Price:$549 CAD