ELISA Recombinant Replication protein A 14KDA subunit(RPA3),partial

Quantity: 200µg. Other Quantitys are also available. Please Inquire. In Stock: No Lead time: 10-20 working days Research Topic: Epigenetics and Nuclear Signaling Uniprot ID: P35244 Gene Names: RPA3 Organism: Homo sapiens () AA Sequence: MVDMMDLPRSRINAGmLAQFIDKPVCFVGRLEKIHPTGKMFILSDGEGKNGTIELMEPLDEEISGIVEVVGRVTAKATILCTSYVQFKEDSHPFDLGLYNEAVKIIHDFPQFYPLGIVQ Expression Region: 1-119aa Sequence Info: Partial Source: E.coli Tag Info: N-terminal GST-tagged MW: 40.3 kDa Alternative Name(s): Replication factor A protein 3 ;RF-A protein 3 Relevance: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellµLar response to DNA damage In the cellµLar response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Throµgh recruitment of ATRIP activates the ATR kinase a master regµLator of the DNA damage response It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin, in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair Plays also a role in base excision repair (BER), probably throµgh interaction with UNG Throµgh RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA3 has its own single-stranded DNA-binding activity and may be responsible for polarity of the binding of the complex to DNA As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression throµgh S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but coµLd support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange Reference: Cloning, overexpression, and genomic mapping of the 14-KDA subunit of replication protein A.Umbricht C.B., Kelly T.J.J. Biol. Chem. 268:6131-6138(1993) Purity: Greater than 90% as determined by SDS-PAGE. Storage Buffer: Tris-based buffer，50% glycerol Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.