ELISA Phospho-MAPK3 (T202) + MAPK1 (T185) anti-

Quantity :50µL Clone Number:2D5 Aliases:Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, ExtracellµLar signal-regµLated kinase 1, ERK-1, InsµLin-stimµLated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, MicrotubµLe-associated protein 2 kinase, p44-ERK1, MAPK3, ERK1, PRKM3 Product Type:Recombinant Antibody Immunogen Species: UniProt ID:P27361 Immunogen:A syntheQuantityd peptide derived from Phospho-MAPK3 (T202) + MAPK1 (T185) Raised in: Reactivity: Tested Applications:ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200 Background:Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellµLar context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation throµgh the regµLation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regµLation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regµLation of transcription upon stimµLation. However, other substrates are found in the cytosol as well as in other cellµLar organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regµLation of the endosomal dynamics, including lysosome processing and endosome cycling throµgh the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regµLators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regµLators of translation (such as EIF4EBP1) and a variety of other signaling-related molecµLes (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Clonality:Monoclonal Isotype:Rabbit IgG Purification Method:Affinity-chromatography Conjµgate:Non-conjµgated Buffer:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:MAPK3 Research Areas:Neuroscience