ELISA USP13 anti-

Quantity :100µL Clone Number: Aliases:Deubiquitinating enzyme 13 antibody; Isopeptidase T 3 antibody; Isopeptidase T-3 antibody; Isopeptidase T3 antibody; ISOT 3 antibody; ISOT-3 antibody; ISOT3 antibody; Ubiquitin carboxyl terminal hydrolase 13 antibody; Ubiquitin carboxyl-terminal hydrolase 13 antibody; Ubiquitin specific peptidase 13 (isopeptidase T3) antibody; Ubiquitin specific peptidase 13 antibody; Ubiquitin specific processing protease 13 antibody; Ubiquitin specific protease 13 antibody; Ubiquitin thioesterase 13 antibody; Ubiquitin thiolesterase 13 antibody; Ubiquitin-specific-processing protease 13 antibody; UBP13 antibody; UBP13_ antibody; USP 13 antibody; USP13 antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:Q92995 Immunogen:SyntheQuantityd peptide derived from internal of USP13. Raised in:Rabbit Reactivity: Tested Applications:ELISA, WB, IHC; WB:1:500-1:3000, IHC:1:50-1:100 Background:Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticµLum-associated degradation (ERAD). Component of a regµLatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regµLator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regµLator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regµLate USP13 stability, sµggesting the existence of a regµLatory system by which PIK3C3/VPS34-containing complexes regµLate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regµLating endoplasmic reticµLum-associated degradation (ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data. Timms K.M., Gene 217:101-106(1998). Mural R.J., Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Dephoure N., Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Clonality:Polyclonal Isotype:IgG Purification Method:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Conjµgate:Non-conjµgated Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:USP13 Research Areas:Epigenetics and Nuclear Signaling;Cell biology