ELISA sdeA anti-

Quantity :50µL Clone Number: Aliases:sdeA antibody; lpg2157 antibody; Ubiquitinating/deubiquitinating enzyme SdeA antibody; Effector protein SdeA) [Includes: Deubiquitinase antibody; DUB antibody; EC 3.4.22.- antibody; Deneddylase antibody; Deubiquitinating enzyme); Ubiquitin transferase antibody; EC 2.3.2.-); Mono-ADP-ribosyltransferase antibody; mART antibody; EC 2.4.2.31)] antibody Product Type:Polyclonal Antibody Immunogen Species:Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) UniProt ID:Q5ZTK4 Immunogen:Recombinant Legionella pneumophila subsp Ubiquitinating/deubiquitinating enzyme SdeA protein (19-268AA) Raised in:Rabbit Reactivity:Legionella pneumophila subsp Tested Applications:ELISA, WB; Recommended dilution: WB:1:1000-1:5000 Background:Secreted effector that interferes with the host cell ubiquitin pathway and is required for intracellµLar bacterial replication. Catalyzes the ubiquitination of several mammalian Rab proteins (Rab33b, Rab1, Rab6a and Rab30) during L.pneumophila infection, without engaging the standard cellµLar enzyme cascade (E1 and E2). Transfers an ADP-ribose moiety from NAD to the \'Arg-42\' residue of ubiquitin in a reaction that releases nicotinamide. The modified ubiquitin is subsequently transferred to the substrate protein throµgh an unknown mechanism that resµLts in the release of AMP. Cannot ubiquitinate the endosomal Rab5 or the cytoskeletal small GTPase Rac1 (PubMed:27049943). Also acts as a deubiquitinase (DUB), catalyzing the cleavage of three of the most abundant polyubiquitin chains (\'Lys-11\', \'Lys-48\' and \'Lys-63\') with a distinct preference for \'Lys-63\' linkages; is thus able to efficiently remove \'Lys-63\'-linked polyubiquitin chains from the phagosomal surface. Is also able to remove NEDD8 from neddylated proteins, but is unable to recognize SUMO. The DUB activity of SdeA is important for regµLating the dynamics of ubiquitin association with the bacterial phagosome, but is dispensable for its role in intracellµLar bacterial replication (PubMed:26598703). Clonality:Polyclonal Isotype:IgG Purification Method:>95%, Protein G purified Conjµgate:Non-conjµgated Buffer:Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:sdeA Research Areas:Others