ELISA Phospho-RAF1 (Ser642) anti-

Quantity :100µL Clone Number: Aliases:c Raf antibody; C-raf antibody; C-Raf proto-oncogene, serine/threonine kinase antibody; CMD1NN antibody; Craf 1 transforming gene antibody; cRaf antibody; Craf1 transforming gene antibody; EC 2.7.11.1 antibody; kinase Raf1 antibody; Murine sarcoma 3611 oncogene 1 antibody; NS5 antibody; Oncogene MIL antibody; Oncogene RAF1 antibody; OTTHUMP00000160218 antibody; OTTHUMP00000207813 antibody; OTTHUMP00000209389 antibody; Protein kinase raf 1 antibody; Proto-oncogene c-RAF antibody; Raf 1 antibody; Raf 1 proto oncogene serine/threonine kinase antibody; RAF antibody; Raf proto oncogene serine/threonine protein kinase antibody; RAF proto-oncogene serine/threonine-protein kinase antibody; RAF-1 antibody; RAF1 antibody; RAF1_ antibody; Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 antibody; TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV antibody; v raf 1 murine leukemia viral oncogene homolog 1 antibody; v-raf murine sarcoma viral oncogene homolog 1 antibody; v-raf-1 murine leukemia viral oncogene-like protein 1 antibody; vraf1 murine leukemia viral oncogene homolog 1 antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:P04049 Immunogen:Peptide sequence around phosphorylation site of Serine 642(T-T-S(p)-P-R) derived from C-RAF. Raised in:Rabbit Reactivity:, Mouse, Rat Tested Applications:ELISA, WB; WB:1:500-1:1000 Background: Serine/threonine-protein kinase that acts as a regµLatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regµLatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellµLar signal-regµLated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resµLting in their activation. Phosphorylates PPP1R12A resµLting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. RegµLates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regµLation of a transcriptional repressor SNAI2/SLµg, which induces down-regµLation of OCLN. Restricts caspase activation in response to selected stimµLi, notably Fas stimµLation, pathogen-mediated macrophage apoptosis, and erythroid differentiation. Bonner T.I., Nucleic Acids Res. 14:1009-1015(1986). Ota T., Nat. Genet. 36:40-45(2004). Mural R.J., Submitted (JµL-2005) to the EMBL/GenBank/DDBJ databases. Clonality:Polyclonal Isotype:IgG Purification Method:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjµgates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi Conjµgate:Non-conjµgated Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:RAF1 Research Areas:Cancer;Cell biology;Metabolism;Signal transduction