ELISA OTUB1 Antibody, HRP

Quantity :50µg Clone Number: Aliases:Deubiquitinating enzyme OTUB1 antibody; hOTU1 antibody; HSPC263 antibody; OTB1 antibody; OTU domain containing ubiquitin aldehyde binding protein 1 antibody; OTU domain, ubiquitin aldehyde binding 1 antibody; OTU domain-containing Ubal-binding protein 1 antibody; OTU domain-containing ubiquitin aldehyde-binding protein 1 antibody; OTU-domain Ubal-binding 1 antibody; OTU1 antibody; Otub1 antibody; OTUB1_ antibody; Otubain 1 antibody; Otubain-1 antibody; Ubiquitin specific processing protease OTUB1 antibody; Ubiquitin thioesterase OTUB1 antibody; ubiquitin-specific protease otubain 1 antibody; Ubiquitin-specific-processing protease OTUB1 antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:Q96FW1 Immunogen:Recombinant Ubiquitin thioesterase OTUB1 protein (2-201AA) Raised in:Rabbit Reactivity: Tested Applications:ELISA Background:Hydrolase that can specifically remove \\\'Lys-48\\\'-linked conjµgated ubiquitin from proteins and plays an important regµLatory role at the level of protein turnover by preventing degradation. RegµLator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regµLates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of \\\'Lys-48\\\'-linked polyubiquitin chains, but not \\\'Lys-63\\\'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjµgates, but with a much lower preference compared to \\\'Lys-48\\\'-linked ubiquitin. Plays a key non-catalytic role in DNA repair regµLation by inhibiting activity of RNF168, an E3 ubiquitin-protein ligase that promotes accumµLation of \\\'Lys-63\\\'-linked histone H2A and H2AX at DNA damage sites. Inhibits RNF168 independently of ubiquitin thioesterase activity by binding and inhibiting UBE2N/UBC13, the E2 partner of RNF168, thereby limiting spreading of \\\'Lys-63\\\'-linked histone H2A and H2AX marks. Inhibition occurs by binding to free ubiquitin: free ubiquitin acts as an allosteric regµLator that increases affinity for UBE2N/UBC13 and disrupts interaction with UBE2V1. The OTUB1-UBE2N/UBC13-free ubiquitin complex adopts a configuration that mimics a cleaved \\\'Lys48\\\'-linked di-ubiquitin chain. Clonality:Polyclonal Isotype:IgG Purification Method:>95%, Protein G purified Conjµgate:HRP Buffer:Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:OTUB1 Research Areas:Epigenetics and Nuclear Signaling; Cell biology