ELISA XRCC5 anti-

Quantity :50µg Clone Number: Aliases:86 kDa subunit of Ku antigen antibody; ATP dependent DNA helicase 2 subunit 2 antibody; ATP dependent DNA helicase II 80 kDa subunit antibody; ATP dependent DNA helicase II 86 Kd subunit antibody; ATP dependent DNA helicase II antibody; ATP-dependent DNA helicase 2 subunit 2 antibody; ATP-dependent DNA helicase II 80 kDa subunit antibody; CTC box binding factor 85 kDa antibody; CTC box-binding factor 85 kDa subunit antibody; CTC85 antibody; CTCBF antibody; DNA repair protein XRCC5 antibody; KARP 1 antibody; KARP1 antibody; Ku 80 antibody; Ku autoantigen 80kDa antibody; Ku80 antibody; Ku86 antibody; Ku86 autoantigen related protein 1 antibody; KUB 2 antibody; KUB2 antibody; Lupus Ku autoantigen protein p86 antibody; NFIV antibody; Nuclear factor IV antibody; Thyroid lupus autoantigen antibody; Thyroid-lupus autoantigen antibody; TLAA antibody; X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody; X-ray repair cross-complementing protein 5 antibody; Xray repair complementing defective repair in Chinese hamster cells 5 antibody; XRCC 5 antibody; XRCC5 antibody; XRCC5_ antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:P13010 Immunogen:Recombinant X-ray repair cross-complementing protein 5 protein (251-455AA) Raised in:Rabbit Reactivity: Tested Applications:ELISA, WB, IHC, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 Background:Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3\\\'-5\\\' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regµLatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together (PubMed:12145306, PubMed:20383123, PubMed:7957065, PubMed:8621488). The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression (PubMed:20383123). The XRCC5/6 dimer probably also acts as a 5\\\'-deoxyribose-5-phosphate lyase (5\\\'-dRP lyase), by catalyzing the beta-elimination of the 5\\\' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5\\\'-dRP activity, and allows to \\\'clean\\\' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regµLator of transcription (PubMed:8621488). Clonality:Polyclonal Isotype:IgG Purification Method:>95%, Protein G purified Conjµgate:Non-conjµgated Buffer:Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:XRCC5 Research Areas:Epigenetics and Nuclear Signaling