ELISA Vimentin mouse monoclonal anti-

Quantity:100µL Host:Mouse Clonality:Monoclonal Isotype:IgG1 GeneName:VIM GeneID:7431 Uniprot:P08670 Specificity:Vimentin antibody detects endogenous levels of total Vimentin. Immunogen:Purified recombinant fragment of Vimentin expressed in E. Coli. Storage:Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. Reactivity:,Mouse,Rat,Monkey Prediction: Application:WB,IHC,IF/ICC,ELISA Dilution:ELISA 1:10000, WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200 Conjµgate:Unconjµgated RRID:AB_2847777 An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site. Cat# Blocking Peptide: Des# Blocking Peptide: Blocking Peptide Price:$549 CAD