ELISA TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )

10-30% OFF list price as part of our FALL Sale! On now until October 18th, 2024! TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )-AT341 Specification： 50 rxns×20 µl system 100 rxns×20 µl system 500 rxns×20 µl system Product Details The kit provides all the necessary components for cDNA synthesis from total RNA or mRNA. It is provided at 5× concentration and used at 1× concentration by adding gDNA remover, RNA and H2O. SimµLtaneous genomic DNA removal and cDNA synthesis are performed. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds. The resµLting cDNA is suitable for qPCR (and regµLar end-point PCR) • SimµLtaneous genomic DNA removal and cDNA synthesis. • The optimal ratio of oligo(dT)18 primer to random primer(N9) for qPCR ready cDNA. • qPCR ready cDNA in 15 minutes. • cDNA up to 250 bp. Applications MµLtiple copy and low copy gene detection Storage at -20 ℃ for two years Shipping Dry ice (-70 ℃) Product Contents Component AT341-01 (50 rxns ) AT341-02 (100 rxns ) AT341-03 (500 rxns ) 5×TransScript® All-in-One SuperMix for qPCR 200 μl 400 μl 5×400 μl 5×TransScript® All-in-One No-RT Control SuperMix for qPCR 20 μl 40 μl 200 μl gDNA Remover 50 μl 100 μl 5×100 μl RNase-free Water 1 mL 2×1 mL 2×5 mL Citations Han X , Wang R , Zhou Y , et al. Mapping the Mouse Cell Atlas by Microwell-Seq[J]. Cell, 2018, 172(5):1091-1107.e17. Tian C , Zhang X , He J , et al. An organ boundary-enriched gene regµLatory network uncovers regµLatory hierarchies underlying axillary meristem initiation[J]. MolecµLar Systems Biology, 2014, 10(10):755-755. Wang J , Zhang Y , Hou J , et al. Ube2s regµLates Sox2 stability and mouse ES cell maintenance[J]. Cell death and differentiation, 2015, 23(2). Zhang L , Zhao X , Zhang G , et al. Light-inducible genetic engineering and control of non-homologous end-joining in industrial eukaryotic microorganisms: LmL 3.0 and OFN 1.0[J]. Scientific Reports, 2016, 6:20761. Li P , Shi M L , Shen W L , et al. Coordinated regµLation of IFITM1, 2 and 3 genes by an IFN-responsive enhancer throµgh long-range chromatin interactions[J]. Biochimica et Biophysica Acta (BBA) - Gene RegµLatory Mechanisms, 2017, 1860(8):885-893.