ELISA TransStart® FastPfu Fly DNA Polymerase

TransStart® FastPfu Fly DNA Polymerase is a hot start, high-fidelity and high processivity DNA Polymerase used for fast PCR. Compared with TransStart® FastPfu DNA Polymerase, TransStart® FastPfu Fly DNA Polymerase has higher extension rate (6kb/min vs 4kb/min), featuring higher amplification efficiency, higher yield, higher fidelity and higher specificity.2×TransStart® FastPfu Fly Reaction Mix already contains dNTPs. When DNA is amplified, just add template, primer, water and TransStart® FastPfu Fly DNA Polymerase. The Reaction can be carried out with the concentration of Reaction Mix being 1×. PCR products are blunt end and can be cloned directly into pEASY®-Blunt series of vectors. • Offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase. • PCR products can be directly cloned into pEASY®-Blunt vectors. • Amplification of genomic DNA fragment up to 15 kb. • Amplification of plasmid DNA fragment up to 20 kb. Applications • Hot start, high specificity. • High amplification efficiency. • Fast and high fidelity. • High sensitivity. • High production. Storage at -20 ℃ for two years Shipping Dry ice (-70 ℃) Product Contents Component AP231-21-V3 AP231-22-V3 AP231-23-V3 TransStart® FastPfu Fly DNA Polymerase 250 U×1 500 U×1 500 U×6 2×TransStart® FastPfu Fly Reaction 1 mL×3 1 mL ×6 1 mL ×36 50 mM MgSO4 200 μl×1 400 μl ×1 1 mL ×1 PCR StimµLant 200 μl×1 400 μl×1 1 mL ×1 6×DNA Loading Buffer 500 μl×1 1 mL ×1 1 mL ×2 Citations Zhu X J , Sun S , Xie B , et al. Guanine-rich sequences inhibit proofreading DNA polymerases[J]. Scientific Reports, 2016, 6:28769. Ye F , Lan X E , Zhu W B , et al. Mitochondrial genomes of praying mantises (Dictyoptera, Mantodea): rearrangement, duplication, and reassignment of tRNA genes[J]. Scientific Reports, 2016, 6:25634. Hu L , Li H , Qin R , et al. Plant phosphomannose isomerase as a selectable marker for rice transformation[J]. Scientific Reports, 2016, 6(1):25921. Chao H , Zhou N Y GenR, an IclR-Type RegµLator, Activates and Represses the Transcription of gen Genes Involved in 3-Hydroxybenzoate and Gentisate Catabolism in Corynebacterium glutamicum[J]. Journal of Bacteriology, 2013, 195(7):1598-1609. Liu X, Pu J, Zeng S, et al. Hyriopsis cumingii Hic52-A novel nacreous layer matrix protein with a collagen-like structure[J].Int J Biol Macromol. 2017 Sep;102:667-673.